CD15 antibody

Catalog No. ABIN112000

This Mouse Monoclonal antibody specifically detects CD15 in FACS, IHC (fro) and CyTox. It exhibits reactivity toward Human.

Quick Overview for CD15 antibody (ABIN112000)

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD15 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Cytotoxicity Test (CyTox)

Clone: VIM-C6

Product Details anti-CD15 Antibody

Purification: Affinity chromatography.

Immunogen: Human granulocytes.

Isotype: IgM

Application Details

Application Notes: ELISA. Immunohistochemistry on frozen sections: 1 μg/mL (1: 200) for tissue staining. Antigen Distribution: Isolated cells: VIM-C6 is heterogenously expressed on granulocytes and on monoblastoidprecursor cells. Tissue sections: VIM-C6 is positive on myeloid precursor cells in the bone marrow. Crossreactions with glandular epithelial cells, astrocytes and Sternberg-Reed cells were reportedfor CD 15.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 0.2 mg/mL

Buffer: PBS buffer pH 7.2 with 0.02 % sodium azide as preservative and 10 mg/mL BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2-8 °C (lyophilized) or reconstituted in aliquots at -20 °C.

Target Details for CD15

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Alternative Name: CD15

Background: CD15 is a carbohydrate antigen expressed mainly on mature granulocytes and monocytes. CD15 is also called Lewis X, X-hapten, lacto-N-fucopentaose III or stage-specific embryonic antigen (SSEA). The reactivity is preferentially with granulocytes and to a lesser degree with monocytes. CD15 is expressed on immature bone marrow cells and leukemic cells of the myelo-monocytic lineage, and sometimes on lymphocytic leukemia cells. VIM-C6 facilitates the differentiation of granulocytes from other blood cells. It is also useful for phenotyping cell subpopulations in myeloic leukemias and for enrichment or elimination of myeloid cells. This antibody reacts with granulocytes, monoblastoid precursor cells.Synonyms: Lacto-N-Fucopentaose III, Lewis X, SSEA1, Stage-Specific Embryonic Antigen, X-Hapten